TUM – Technical University of Munich Menu
Trypanosomen in der Durchlichtmikroskopie (links) und in der Fluoreszenzmikroskopie (rechts). Angefärbt sind hier die Glykosomen (rot), gegen die sich der Wirkstoff richtet, sowie die DNA des Parasiten (blau). Bild: Ralf Erdmann, Vishal Kalel / Ruhr-Universität Bochum
Trypanosomen in der Durchlichtmikroskopie (links) und in der Fluoreszenzmikroskopie (rechts). Angefärbt sind hier die Glykosomen (rot), gegen die sich der Wirkstoff richtet, sowie die DNA des Parasiten (blau). Bild: Ralf Erdmann, Vishal Kalel / Ruhr-Universität Bochum
  • Research news

Structural Biology develops active agent that blocks trypanosomes

New therapeutic strategy against sleeping sickness

A newly developed small molecule selectively kills the pathogen causing sleeping sickness and Chagas disease. Scientists from the Technical University of Munich, the Helmholtz Zentrum München and from the Ruhr University Bochum first determined the parasite's Achilles heel by using modern structural biology techniques and then developed an inhibitor with a perfect fit.

Trypanosomes get their name from the Greek trypano- (borer) and soma (body). The protozoan parasites are responsible for various diseases, particularly in Latin America and Africa. The best known example is probably sleeping sickness, which is caused by trypanosomes and transmitted by tsetse flies. In the end stage, patients suffer from uncontrollable sleep, which gave the sickness its name.

A further example is the so-called Chagas disease, which is caused by Trypanosoma cruzi and transmitted by so-called kissing bugs (Triatominae). Around eight million people are estimated to be affected in North and South America. Symptoms include heart problems, as well as nerve damage in the digestive tract. In the current study, Trypanosoma cruzi was examined as a representative.

“Until now, there have been only a few medications against trypanosomes. These drugs have many undesirable side effects, and first cases of resistance are already spreading,” explains Prof. Michael Sattler, Director of the Institute of Structural Biology at the Helmholtz Zentrum München and Professor of Biomolecular NMR Spectroscopy at the Technical University of Munich.

Together with Dr. Grzegorz Popowicz (also Helmholtz Zentrum München) and the group headed by Prof. Ralf Erdmann at Ruhr University Bochum, the research team sought new possibilities to deactivate the pathogen. “We primarily concentrated on the so-called PEX proteins, which have been under debate as potential targets for pharmacological interference for some time,” says Sattler.

PEX proteins are the key

The PEX proteins play a crucial role in the function of the so-called glycosomes. These are small cell organelles that are important to maintain the parasite’s sugar metabolism. “The idea was to prevent the interaction between two essential proteins, PEX14 and PEX5, and consequently to disrupt the trypanosome metabolism so effectively that the parasites cannot survive,” explains Grzegorz Popowicz.

At the Bavarian NMR Center, a joint research infrastructure of the Helmholtz Zentrum München and TU Munich, the researchers therefore first used nuclear magnetic resonance (NMR) measurements to investigate the structure of the two target proteins.

In the next step, the teams from Munich and Bochum used the thus determined spatial structure to optimize a substance that specifically binds to PEX14, thereby preventing the interaction with PEX5, which eventually kills the parasite. Grzegorz Popowicz describes it as, "We more or less first measured the lock and then developed the right key for it." .

Possibly also relevant for other parasites.

In the future, the researchers want to further advance these molecules using medicinal chemistry so that they can be tested in clinical studies and, eventually may be approved as drugs. They are also examining the extent to which the approach is suitable to kill other single-cell parasites that may depend on similar proteins. “One possibility would be to target the Leishmania parasite,” explains Popowicz. Future research in this direction will follow.


The work was funded by the European Union (Marie Sklodowska-Curie program), the Deutsche Forschungsgemeinschaft (e.g., Cluster of Excellence Center for Integrated Protein Science Munich) and the Helmholtz Association, the Wroclaw Centre of Biotechnology and the Ministry of Education, Youth and Sport of the Czech Republic. X-ray structure analysis measurements were carried out at the European Synchrotron Radiation Facility (Grenoble, France), NMR measurements at the Bavarian NMR Center in Garching (Germany). Further participants of the study were the Central European Institute of Technology of the Masaryk University (Brno, Czech Republic), the Swiss Tropical and Public Health Institute and the University of Basel (Basel, Switzerland) as well as the New York University School of Medicine (New York, USA).


Inhibitors of PEX14 disrupt protein import into glycosomes and kill Trypanosoma parasitesM. Dawidowski, L. Emmanouilidis, V. C. Kalel, K. Tripsianes, K. Schorpp, K. Hadian, M. Kaiser, P. Mäser, M. Kolonko, S. Tanghe, A. Rodriguez,W. Schliebs, R. Erdmann, M. Sattler, G. M. Popowicz
Science, March 31, 2017, Vol. 355, Issue 6332, pp. 1416-1420 – DOI: 10.1126/science.aal1807


Prof. Dr. Michael Sattler
Technical University of Munich
Chair of Biomolecular NMR Spectroscopy
Lichtenbergstr. 4, 85748 Garching, Germany
Tel.: +49 89 289 13418E-MailWeb

Corporate Communications Center

Technical University of Munich

Article at tum.de

Im Programm TUM DesignBuild der Fakultät für Architektur planen Studierende Projekte im sozialen Kontext und setzen sie mit der Bevölkerung vor Ort um. Diese Modell-Schule in Sambia entstand in Zusammenarbeit des Lehrstuhls für Holzbau und Baukonstruktion, des Lehrstuhls für Entwerfen und Holzbau und der University of Zambia. (Bild: Matthias Kestel)

TUM starts long-term Africa initiative

The Technical University of Munich (TUM) has launched an Africa initiative. In addition to collaboration in individual projects, in the future long-term partnerships in the key areas teaching, research and entrepreneurship...

Das Protein (blau) erkennt die pri-miR18a (pink) und formt deren Struktur zur fertigen miRNA. (Bild: H. Kooshapur / TUM)

New Insights into the Maturation of miRNAs

An international research team has used a structural biological approach to elucidate the maturation of a cancer-causing microRNA in gene regulation. In the future, the authors hope to develop new therapies based on their...

Leibniz-Preisträger Prof. Franz Pfeiffer und eine Wissenschafterin arbeiten an einem Projekt mit dem Mini-Teilchenbeschleuniger MuCLS.

Munich School of BioEngineering sets European standards

Two years after its founding at the Technical University of Munich (TUM), the Munich School of BioEngineering (MSB) is expanding its scope of action: The upcoming opening of the MSB-associated Central Institute for...

Bindung der großen Untereinheit von U2AF an die Vorläufer-Boten-mRNA – Bild: Christoph Hohmann / NIM

Shaping up to make the cut

Before RNA transcripts of genes can program the synthesis of proteins, the non-coding regions are removed by the spliceosome, a complex molecular machine. The correct regulation of the splicing plays a central role for many...

Fasern des Muskelproteins Aktin

TU München establishes center for protein research

Technische Universität München (TUM) is establishing the “TUM Center for Functional Protein Assemblies (CPA)” to concentrate its wide-ranging expertise in protein research. It will conduct cross-departmental research into...