Dr. Valentin Riedl (left), research group leader in the Neuroradiology Department of University Hospital rechts der Isar of the TUM, with his colleague Dr. Christian Sorg. (Image: K. Bauer / TUM)
Dr. Valentin Riedl (left), research group leader in the Neuroradiology Department of University Hospital rechts der Isar of the TUM, with his colleague Dr. Christian Sorg. (Image: K. Bauer / TUM)

Interplay of neurotransmitters in the brain in response to optical stimuli explainedSeeing begins before we actually see anything

How does vision work, and what happens in the brain during the process? As simple as this question may sound, it has yet to be scientifically clarified in full. Dr. Valentin Riedl of the Technical University of Munich (TUM) and his team have now been able to show that the distribution of the two most important neurotransmitters in the brain changes as soon as we open our eyes, regardless of whether we actually see anything.

To communicate with each other, neurons use chemical messengers known as neurotransmitters. The two most important neurotransmitters in the human brain, glutamate and GABA, have opposing effects: glutamate activates neurons, while GABA suppresses them. Glutamate, incidentally, is also used as spicy substance and can be found in tomatoes and Parmesan cheese. By altering the concentrations of the two neurotransmitters, the brain is able to process impressions from the eyes, called visual stimuli.

Privatdozent Dr. Valentin Riedl, research group leader in the Neuroradiology Department of University Hospital rechts der Isar of the TUM, and his team have studied how the concentrations of the two neurotransmitters change in the visual cortex, the region in the brain responsible for vision. The study is unique in that the team used magnetic resonance spectroscopy (MRS) to measure the concentrations of the neurotransmitters in detail and, above all, in parallel.

Visual process triggered by opening the eyes

The experiment consisted of three phases. The subjects first lay in the dark for five minutes with their eyes closed. They then opened their eyes and stared into the darkness. Finally, they were shown a checkerboard pattern that blinked on and off rapidly. Throughout the experiment, the concentrations of both neurotransmitters in the visual cortex were measured simultaneously.

In the resting state with the eyes closed, GABA levels were high. Surprisingly, however, concentrations of this inhibitory neurotransmitter decreased as soon as the subjects opened their eyes, despite the fact that there was still nothing to see. “The brain prepares for forthcoming stimuli as soon as the eyes are opened. This phenomenon had never previously been observed, because other studies had not measured this state,” Riedl says. Only when an actual visual stimulus was perceived, i.e. the blinking checkerboard pattern, did the concentration of glutamate, the activating neurotransmitter, increase.

Data consistent with fMRT measurements

For the first time, the researchers also compared their MRS data with data obtained by functional MRI (fMRI), a common method for visualizing human brain activity. In this technique, the consumption of oxygen is measured in specific brain regions. A high consumption is an indirect indicator of neuronal activity in a given area.

They found that changes in neurotransmitter levels in the visual cortex coincided with evidence of brain activity in the fMRI scans. “The results of the two methods agreed perfectly. By combining the two techniques, we’re not only able to say that there is increased activity in a region; for the first time we’re also able to specifically attribute that activity to the two neurotransmitters,” Riedl explains.

Psychiatric disorders as a research field

The findings by Riedl and his team also have clinical relevance. For example, it is suspected that the distribution of the two neurotransmitters is permanently disturbed in psychiatric disorders such as schizophrenia. “To date, however, there is no proof of this. An examination using both spectroscopy and fMRT would provide much more precise and far-reaching information on the concentrations of the neurotransmitters in patients,” Riedl says.


Katarzyna Kurcyus, Efsun Annac, Nina M. Hanning, Ashley D. Harris, Georg Oeltzschner, Richard Edden and Valentin Riedl, Opposite dynamics of GABA and glutamate levels in the occipital cortex during visual processing, Journal of Neuroscience, November 14, 2018, DOI: 10.1523/JNEUROSCI.1214-18.2018


PD Dr. Valentin Riedl
Neuroimaging Center at University Hospital rechts der Isar of the TUM
Tel.: +49 (0)89 4140 - 7972

More information

Download high-resolution image


Corporate Communications Center

Technical University of Munich Dr. Vera Siegler

Article at tum.de

Was führt zu der Entscheidung, wohin jemand schaut, wenn nichts Bestimmtes gesucht wird und zunächst unklar ist, wohin sich der Blick richten sollte? (Foto: iStockphoto/ praetorianphoto)

What catches our eye

Our unconscious gaze is controlled by an automatic selection process computed by a neural network in the brain. Details of this computation have now been studied  by an international team collaborating with the Technical...

Patienten mit Restless Legs Syndrom verspüren nachts einen starken Bewegungsdrang und leiden an unangenehmen Empfindungen wie Schmerzen oder Kribbeln in den Beinen. (Bild: burakkarademir / iStock)

Restless legs syndrome: New genetic risk variants found

Restless legs syndrome (RLS) is characterized by restless, painful legs that do not settle down at night. The causes are largely unknown. An international team led by the Technical University of Munich (TUM) and the...

Josef Rauschecker und Markus Ploner.

Ringing in the ears and chronic pain enter by the same gate

Tinnitus and chronic pain have more in common than their ability to afflict millions with the very real experience of "phantom" sensations. Scientists noted similarities between the two disorders more than thirty years ago....

Das Bild zeigt die EEG-Ergebnisse während eines kurzen (links) und eines langandauernden Schmerzreizes (rechts). Die Hirnbereiche mit der stärksten Aktivität erscheinen rot. Kurzer Schmerz wird in sensorischen Bereichen verarbeitet, langandauernder Schmerz eher in emotionalen Hirnbereichen in der Stirnregion. (Bild: E. Schulz et al., 2015, Prefrontal gamma oscillations encode tonic pain in humans, Cerebral Cortex, doi10.1093/cercor/bhv043, modifiziert)

Brain processes ongoing pain more emotionally

A momentary lapse of concentration is all it takes for a finger to become trapped, to receive a bump on the head or sprain an ankle – and it hurts. Pain is the body’s indispensable protective mechanism, and at the same...